Record the data of "BLAST"

Result:

Test	Date	Observation
Gram stain+	10-9-18	Gram positive rods
Endospore+	10-18-18	Endospore present
KOH-	10-25-18	No string visible
Catalase+	10-25-18	Bubbles were present
Motility-	10-25-18	Growth confined to the stab line
Aerobic growth+	10-30-18	Bacteria growth
Anaerobic growth+	10-30-18	Bacteria growth
L- Arabinose- Starch -	10-30-18 10-30-18	No changes in color Observation????
D-Glucose-	11-1-18	No change in color
Glycogen+	11-5-18	Color change to yellowish
D-Mannose-	11-5-18	No changes in color
D-Xylose-	11-5-18	No changes in color
Growth at pH 5 -	11-13-18	No growth indicated/change color after 24 hrs
Growth at pH 10 +	11-13-18	Turbidity in liquid medium
Growth at NaCl 2% +	11-13-18	Turbidity in liquid medium
Growth at NaCl 10% -	11-13-18	No growth indicated/change color after 24 hrs
Growth at pH 6 +	11-15-18	Turbidity in liquid medium
Growth at pH 9-	11-15-18	No growth indicated/change color after 24 hrs
Growth at NaCl 7% -	11-15-18	No growth indicated/change color after 24 hrs
Citrate +	11-15-18	Color change from green to blue

Th 23B labeled unknown bacteria had the following characteristic on the TSA plate: different sizes in an opaque creamy color. Colonies have round shape and it smells like stagnant water from the fish tank. After the gram stain were performed, there were purple rod seen under the microscope and little pink on the side. After confirming that it was gram positive, the KOH and catalase were performed to double checked the result. For the KOH, there is no string visible that confirmed it was gram positive. For the catalase test bubbles were present and it indicating positive gram. Endospore were present and indicated that 23B was Gram positive rods Bacillus.

Various tests were conducted on each the unknown bacterial cultures. An explanation of each test and the results are in table. The tests were performed in such a way that each test eliminated a possible unknown candidate.