cell biology lab report on LAB 1A: DNA Isolation from Wheat Germ LAB 1B: Spectrophotometric Quantification of DNA and Protein

physical disruption of tissues/cells ● Removal of lipids and dissociation of DNP complexes ● Removal of proteins ● Precipitation of DNA Homogenization 5 g wheat germ is blended with 30 mL of warm DNA buffer Centrifugation 10 min @ 4000 x g Discard supernatant Lipid Removal Resuspend in 5 mL DNA buffer + 4 drops Triton-X This solubilizes membranes/lipids & results in dissociation of the DNP complexes (separates DNA & associated proteins) Centrifugation 15 min @ 4000 x g Discard supernatant DNA and proteinlipids Protein Removal Resuspend in 30 mL 2.6M NaCl This allows the precipitation of proteins, while the DNA remains in solution Centrifugation 15 min @ 20,000 x g KEEP supernatant proteinDNA Isolate DNA Carefully add 30 mL 70% ethanol This allows the precipitation of DNA at the interface interfaceEtOH salt solution Collect Collect DNA by rapidly stirring and place on plastic Petri dish Clean Remove liquid, wash with 10 mL EtOH, and again remove liquid.

Allow to dry for the following lab (1B) Lab Report ● Lab 1 is to be submitted as a formal lab report (see the General Lab Report Guidelines AND the Lab 1 Report Requirements ) ● Your lab report must be submitted through Nexus by 11:59 PM on October 8 th as a pdf file ● It is worth 7.5% of your final grade! Start Now!

● Introduction : max 750 words – Provides the necessary background information to your reader.

– Start with importance and general purpose – Conclude with brief overview of methodology and specific questions to be asked ● Methods : no word limit – Includes all of the methods utilized for this lab and is written in proper paragraphs – You do not need to describe basic techniques – You must reference the lab manual Avoid Plagiarism!

● Ensure you are familiar with the rules and regulations on plagiarism ● You cannot utilize the same or similar phrasing as any other source! This includes the lab manual. ● All information from another source must be given in your own words and be appropriately referenced .