cell biology lab report on LAB 1A: DNA Isolation from Wheat Germ LAB 1B: Spectrophotometric Quantification of DNA and Protein

Data for Lab 1 Sample information: Dry weight of DNA isolated was 0.0837 grams. Subsample for analysis was prepared by dissolving 30 mg of DNA in30 mL of 0.01 NNaOH. Biuret assay Standard curve Protein concentration Absorbance 0.094 0.005 0.188 0.016 0.375 0.037 0.750 0.105 1.50 0.1 87 3.00 0.3 84 Samples Dilution Absorbance Undiluted 0.02 9 2-Fold 0.01 4 5-Fold 0.00 6 Internal control 0.04 6 Lowry assay Standard curve Protein concentration Absorbance 0.094 0.0 74 0.188 0.144 0.375 0.278 0.750 0.562 1.50 0.724 3.00 0.803 Samples Dilution Absorbance Undiluted 0.163 2-Fold 0.071 5-Fold 0.029 Internal control 0.2 27 Dische assay Standard curve DNA concentration Absorbance 0.0 625 0.094 0.1 25 0.2 16 0.25 0.4 45 0.50 0.897 1.00 1.6 85 Samples Dilution Absorbance Undiluted 0.941 Internal control 0.44 1 Nanodrop analysis: protein Dilution Concentration (μg/μl) Undiluted 0.3 05 2-Fold 0.134 5-Fold 0.0 58 Internal control 0.36 9 Nanodrop analysis: DNA Dilution Concentration (ng/ μl) 260/280 5-Fold 131.9 1.77 10 -Fold 63.2 1.7 9 Internal control 25.1 1.9 2