DifferentialCentrifugationfortheLocalizationofEnzymesandtheExaminationofEnzymeFunction

Lab 3 Assignment: D ifferential C entrifugation for the Localization of Enzymes and the Examination of Enzyme Function Worth: 2% (mark out of 20) Due: October 22 nd,2020 Data Analysis 1. Provide a properly formatted table containing the total volume collected, enzyme units/ml, total enzyme units, and % of enzyme units from the original homogenate for each fraction from Part A.(2marks) 2. Prepare aproperly formatted figure of the standard curve for DCIP concentration. (1 mark) 3. Prepare afigure of the effects on altering enzyme amount on the absorbance due to DCIP reduction. (1.5 marks) 4. Prepare afigure containing data for tubes 6-10 of your Lab 3B data. (2.5 marks) 5. State the maximum rate of succinate dehydrogenase activity (succinate oxidation) for each of your samples from Lab 3B. Include a sample calculation for one of these samples. (4 marks) Questions 1. Based on your results, state all of your samples which contain AP .What does this tell you about the location of AP in the cell? Explain your answer. (2 marks) 2.Based on the maximum rates you calculated, explain your observations for the effect of altering the amount of mitochondrial pellet. (1 mark) 3. Explain your observed maximum rates for each of the alterations in tubes 7-10. Did the rate increase or decrease compared to tube 6? Why was this effect observed? (4 marks) 4. Based on your knowledge of the location of SDH in the cell, in which of the five fractions collected in Part A would you expect to find SDH? Provide all of the fractions in which enzyme activity would be found. (1.5 marks) 5. Ifyou were to perform a final centrifugation on the post-mitochondrial supernatant in order to isolate the microsomal components, would you expect to find SDH activity in the pellet, supernatant, both, or neither? (0.5 marks)