Any tutor excellent in handling any lab questions and reports relating to biochemical and DNA technology.

2020 autumn BIOL S356F Biochemical and DNA Technologies Laboratory Raw data Experiment 1 - Species Identification of Meats by PCR -RFLP Below are images of PCR products of three unknown samples and digested PCR products of three reference meat samples * with 100 bp Plus DNA ladder, run on agarose gel and stained with GelRed * PCR and restriction digestion of reference meat samples were done by staffs Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10 Lane 11 DNA Marker Negative Control PCR Product BsaJI RsaI A B C Beef Chic Pork Beef Chic Pork Reference result obtained from teaching staff s: Result obtained from group X : 2020 autumn Below are images of digested PCR products of three unknown samples with 100 bp Plus DNA ladder, run on polyacrylamide gel and stained with GelRed Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 DNA Marker Enzyme X Enzyme Y Sample A Sample B Sample C Sample A Sample B Sample C Reference result obtained from teaching staffs: Result obtained from group X (using PCR products from another group for digestion ): 2020 autumn Experiment 2 - Detection, Quantitation, and Identification of Target Contaminant in Water Below are tables show ing the absorbance of solution after ELISA at 450nm and 600 nm 1 2 3 4 A Blank Blank Sample 1 original Sample 1 original B Standard 1st dilution* Standard 1st dilution* Sample 1 2-fold dilution Sample 1 2-fold dilution C Standard 2nd dilution Standard 2nd dilution Sample 1 4-fold dilution Sample 1 4-fold dilution D Standard 3rd dilution Standard 3rd dilution Sample 2 original Sample 2 original E Standard 4th dilution Standard 4th dilution Sample 2 2-fold dilution Sample 2 2-fold dilution F Standard 5th dilution Standard 5th dilution Sample 2 4-fold dilution Sample 2 4-fold dilution * The 1 st dilution was prepared by adding 2μL of 1μg/mL of contaminant standard to 798μL diluent buffer ( see step 2 in part D of the procedures) Reference result obtained from teaching staffs: 450nm 1 2 3 4 A 0.092 0.099 1.067 1.019 B 1.052 0.965 0.806 0.813 C 0.799 0.761 0.605 0.618 D 0.585 0.564 0.622 0.615 E 0.456 0.443 0.487 0.467 F 0.398 0.364 0.338 0.385 Result obtained from group X : 450nm 1 2 3 4 A 0.092 0.118 0.129 0.102 B 0.080 0.100 0.109 0.110 C 0.071 0.116 0.123 0.135 D 0.072 0.103 0.101 0.113 E 0.080 0.105 0.109 0.101 F 0.096 0.099 0.104 0.102 600nm 1 2 3 4 A 0.037 0.034 0.036 0.037 B 0.034 0.034 0.035 0.035 C 0.034 0.034 0.035 0.035 D 0.034 0.034 0.034 0.034 E 0.034 0.034 0.036 0.036 F 0.034 0.039 0.034 0.034 600nm 1 2 3 4 A 0.033 0.034 0.036 0.039 B 0.037 0.035 0.035 0.035 C 0.034 0.035 0.036 0.033 D 0.039 0.038 0.036 0.037 E 0.036 0.037 0.038 0.038 F 0.038 0.037 0.037 0.038