HI SIR. FOR LAB REPORT I FOUNF THE URL
2020 Autumn
Special Notes for Laboratory BIOL S356F
1. Time points for PCR
| Session | Time | |
| Collect samples for PCR | Return PCR products | |
| 10 a.m. | 12:30 p.m. | |
| 12:30 p.m. | Beginning of next session | |
| 10 a.m. | 12:30 p.m. | |
| 12:30 p.m. | Beginning of next session | |
| 10 a.m. | 12:30 p.m. | |
| 12:30 p.m. | Beginning of next session | |
For lab sessions from 2 p.m. to 6 p.m.:
| Session | Time | |
| Collect samples for PCR | Return PCR products | |
| 3 p.m. | 5:30 p.m. | |
| 5:30 p.m. | Beginning of next session | |
| 3 p.m. | 5:30 p.m. | |
| 5:30 p.m. | Beginning of next session | |
| 3 p.m. | 5:30 p.m. | |
| 5:30 p.m. | Beginning of next session | |
2. Limited materials given upon request
PCR-RFLP:
Maximum quantity
10uM dNTPs 2*
Forward primer 2*
Reverse primer 2*
Taq polymerase (1U/ul) 2*
Restriction enzyme X and Y 2#
* Each quantity is enough for PCR of 4 samples
# Each quantity is enough for restriction digestion of 3 samples
ELISA:
Maximum quantity
96-well ELISA plate 1
1μg/mL contaminant standard 2+
2 water samples 2
Capture antibody 2^
Primary detection antibody 2^
Conjugated secondary antibody 2^
+ Each quantity is 5μL in a PCR tube
^ Each quantity is enough for dilution in 3mL PBS/ diluent buffer
Remark:
- All other materials will be provided on the teaching bench in a self-service manner
- Take only the quantities required for your experiment
- Keep all storable materials in the storage box provided at the end of every lab session
3. Arrangement for running PAGE
Each PAGE gel tank can run a maximum of 4 gels at the same time and there are 3 gel tanks provided.
As the electrode assembly requires a pair of 2 gels to hold the TBE buffer inside (refer to demo video “PAGE gel casting”), it is suggested that each group finds another group and try best to run their gels together.
