read the template follow instructions as stated. NO plagiarism. Come up with a title

Unknown Template (Arial 12 point font, double spaced, 1 inch margins)

Title Page

Student’s name

Purpose:

Explain why this project was done

Introduction:

Should be a minimum of 2 pages explaining why one would want to identify an unknown bacterium. What tests should be done and why? Use the lab manual, outside sources, and the handouts you’ve been provided for this information. Also if you are taking the lecture as well the textbook has very good information as well. NO PERSONAL PRONOUNS (I, we, us, they, my, etc). In text citations identifying where you obtained information should be used (author, year). NO QUOTES. Write in your own words.

Results:

Test	Observations	Interpretation
Gram Stain	Red or pink rod	Gram negative rod
KOH	Sticky	Gram negative
EMB	Moderate	Gram negative
PEA	No growth	Gram negative
Urea	Yellow	Doesn’t produce urease
PRFerm glucose	Yellow w bubble	Positive for fermentation
PRFerm lactose	Yellow w bubble	Positive for fermentation
PRFerm sucrose	Yellow w bubble	Positive foe fermentation
Methyl Red (MR)	Yellow	Negative for methyl red
Voges Proskauer (VP)	Red	Positive for acetoin
Catalase	Bubbles	Positive for catalase
Oxidase	Yellow	Doesn’t become oxidized
Citrate	Blue	Positive for citrate
PAD	Yellow	No phenylalanine produced
Sulfur	Yellow	Negative for sulfur
Indole	Yellow	Negative doesn’t produce indole
Motility	Fuzzy red	Bacteria produces motile

Do not record interpretation as +/-. For example: If you observe that the urea tube is pink, then the interpretation is that the bacteria produces urease.

Discussion:

Should be a MINIMUM of 2 pages long. Discuss your results and state how you identified your unknown. Hint: Use the flow chart as a guide. Then state: The unknown is:?????

The rest of your discussion should focus on the organism. Does it cause disease, treatments, where is it normally found, etc. In text citations identifying where you obtained information should be used (author, year).

References:

Include references in APA format. Make sure that any included urls open to the publisher’s website and not to a database. All references in the list should also be included as in text citations.