Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you'll need to watch the video to answer the questions. This for the most part is not true. I usual
Watc h th e v id eo a bout P CR ( h ttp s://w ww.jo ve.c om /v /5 056/p cr-th e-p oly m era se-c hain -re actio n) a nd th e o ne a bout N ext G enera tio n S equencin g w it h E ric C how ( h ttp s://w ww.y outu be.c om /w atc h?v= m I0 F o9kaW qo ) A nsw er th e q uestio ns b elo w : 1. W hat is ta q p oly m era se a nd w hy is it u sed in P CR?2. B rie fly d escrib e e ach p hase o f th e P CR th erm ocyclin g:
denatu ra tio n annealin g elo ngatio n 3. W hy a re p rim ers im porta nt fo r P CR?
4. D efin e th e fo llo w in g in re gard s to S anger s equencin g:
dntp flo ure scent te rm in ato rs 5 . W hat a re tw o a dvanta ges th at N ext G enera tio n s equencin g h as o ver tra dit io nal S anger s equencin g?
6. W it h th e Illu m in a a nd s im ila r m achin es, h ow m uch d oes it c ost to s equence a n e ntir e h um an g enom e, a nd in w hat tim e fra m e?
7. H ow m ust D NA s am ple s b e p re pare d to b e in serte d o nto flo w c ells ? S ie rra N etz -A pr 0 6, 2 021, 2 :1 0 P M M DT
