Hello. Attached I have 3 genetic labs that I need assistance with. In some of the questions, it says you'll need to watch the video to answer the questions. This for the most part is not true. I usual

Watc h a t le ast th e fir s t 4 m in ute s o f th is v id eo to le arn a bout th e b asic p rin cip le s o f v ecto r c lo nin g fro m J oV E (s ig n in w it h C NM cre dentia ls ): h ttp s://w ww.jo ve.c om /v /5 074/m ole cula r-c lo nin g a nd th e fir s t 3 m in ute s o f th is v id eo to le arn th e b asic s a bout re stric tio n e nzym es: http s://w ww.jo ve.c om /v /5 070/re stric tio n-e nzym e-d ig ests B oth v id eos g o d eep in to d eta ils a bout th e p ro cesses a nd y ou d on't n eed th at d epth o f in fo rm atio n, ju st th e b asic s.

Y ou a re g oin g to s ee if it m ig ht b e p ossib le to u se a s pecif ic b acte ria l p la sm id , c alle d p U C19, to c lo ne a p ie ce o f th e h um an R FP p ro te in u sin g o ne or m ore re stric tio n e nzym es.

Y ou w ill n eed :

1)th e re fe re nce s equence fo r th e fir s t 1 800 b ases (fro m N CBI B la st D ata base) in th e R FP p ro te in , (a tta chm ent) 2 ) th e lis t o f re stric tio n e nzym es (fo und a t th e b otto m o f th is p age) 3) th e s chem atic fo r b acte ria l c lo nin g p la sm id , p U C19 (a tta chm ent) L ook a t th e re stric tio n e nzym es o n th e lis t th at y ou h ave b een g iv en. T hese w ill a ll c ut s om ew here w it h in th e m ult ip le c lo nin g s it e (M CS) o n pU C19. O ut o f th e lis t, fin d fo ur re stric tio n e nzym es th at w ill c ut s om ew here w it h in th e 1 800 b ase-p air R FP re fe re nce s equence y ou a re g iv en. 1.) T he re fe re nce s equence is lis te d 5 ’- 3 ’. Y ou c an s earc h fo r re stric tio n s it e s o n th is s equence b y u sin g th e “F in d” fu nctio n in y our w ord p ro cessor. U se fo ur d if fe re nt c olo r h ig hlig hts to m ark o n y our R FP re fe re nce g ene s equence a ll th e s it e s w here e ach o f y our re stric tio n enzym es w ill c ut. F or e xam ple , to fin d th e p la ces w here E coR 1 w ould c ut o n th e R FP, y ou w ould c opy th e E coR 1 re stric tio n re cognit io n s it e fro m th e lis t ( G AATTC ) a nd th en p aste it in to th e “fin d” fu nctio n o n y our w ord p ro cessor in th e re fe re nce s equence. E very s it e w here th is s equence is fo und w ill s how u p a nd th en y ou c an h ig hlig ht th em . T ry th is w it h e very re stric tio n s it e lis te d.

2.) N am e o f R E 1.

4. 3.) L ook fo r s om eth in g th at c uts c lo se to th e b egin nin g a nd s om eth in g th at c uts c lo se to th e e nd.

4.) L is t o f r e str ic tio n e n zym e r e co gnitio n s eq uen ces fr o m Wik ip ed ia (a ll a re lis te d in 5 ’  3 ’ d ir e ctio n) a ll o f th ese a re fo und w ith in th e pU C19 M CS: Acc65I- G GTACC A poI- A ATT B spM I- A CCTGC B am HI- G GATCC Sie rra N etz -M ar 2 5, 2 021, 1 0:4 5 A M M DT EcoR I- G AATTC H in dIII- A AGCTT P stI- C TGCAG S alI - G TCGAC S acI- G AGCTC S bfI- C CTGCAGG S m aI- C CCGGG S phI- G CATGC