Do a flowsheet to differentiate these organisms. I have uploaded an example and the assignment document. Gram negative enteric rods: Escherichia coli Klebsiella pneumonia Enterobacter aerogenes Citro
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So, what is a “flowsheet” anyway?
See below the basic idea of a flowsheet. It is all about sequential sub-division to extinction. Before you can proceed with this you must first determine the Gram reaction and cell morphology of your organism (ie. do you have a Gram + cocci, Gram + rod or Gram – rod). This design applies to both the preliminary and final flowsheets. Imagine that the letters (a, b, c….) represent the organisms in your group (Gr+ cocci, etc.). Notice that once a test has been used to separate an organism or group of organisms from the others you DO NOT re-combine these organisms again. Continue to subdivide the smaller groups until each organism in your group has been separated from the others. Remember to follow the instructions in the “Introduction to unknowns” exercise. Remember that you should only separate the organisms in the group that your organism belongs based upon the preliminary characterization (Gram reaction and cell morphology). For each level in your flowsheet, write a few words to indicate the particular test that you are using. This corresponds to the position of the words “test” below. Then include a few words to describe each possible reaction for the test. DO NOT put in the words “positive reaction” or “negative reaction” or their symbols “+” or “-“ for possible reactions. Rather, use specific descriptive terms such as “turns blue” vs “stays green”, or “bubbles” vs “no bubbles.” If you have a question then you should take the initiative to ASK!
a
b
c
d
e
f
g
h
possible reaction Test ______ possible reaction
a d
b e
c f
g
p
ossible reaction Test possible reaction h a c
b possible reaction Test possible reaction
d g
possible reaction Test possible reaction e h
a b f
possible reaction Test possible reaction
g h
possible reaction Test possible reaction
d f
e
possible reaction Test possible reaction
d e
