Please answer all of the following questions carefully. No Plagiarism. Thank You.

1. Based on your results, the DNA sample obtained from the crime scene most probably comes from which suspect? Explain.

2. Now assume that the DNA samples from suspect 1 and suspect 2 were cut only with enzyme 1. Would you be able to come out with the same result and conclusion?

3. What properties of agarose gels make them appropriate to be used for DNA gel electrophoresis?

4. Why is DNA positioned at the black-cathode end? What would happen if you misplace the DNA-containing wells at the anode end?

5. What would happen if you use water instead of TAE buffer? What is the function of TAE buffer during gel running?

6. Why do DNA samples need to be premixed with DNA loading buffer? What are the key functions of this buffer?

7. Why do you need a UV transilluminator to observe your DNA bands?

8. Describe (an)other method(s) used in molecular biology labs to detect DNA (either in gels or membranes).

9. What are Southern and Northern blotting methods?

10. What are the most common applications of restriction enzymes and the DNA recombinant technology? (List at least 3)

11. What are the differences between blunt and sticky ends? Give examples of enzymes that generate each type.

12. How would you clone “your gene of interest” into a bacterial plasmid using KpnI restriction enzyme? Use Figure 19.2 as a reference