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QUESTION

(5pts) Complete the plasmid mapping problem.5/2 Lane 1 2 3 ___ ___ 4 5 6 7 8 ___6.0 ___5.0 ___4.0 ___3.0 ___ ___ ___ ___ ___2.0 ___ ___ ___ ___ ___...

Restriction digest of pJTR1195. 0.5ug of ppJTR1195 was digested with the indicated restriction enzymes and subjected to gel electrophoresis through a 0.75% agarose gel.

lane 1: BamHI, lane 2: HindIII, lane 3: EcoRI, lane 4 BamHI/HindIII,

lane 5: BamHI/EcoRI , lane 6 EcoRI/HindIII , lane 7 EcoRI/BamHI/HindIII

lane 8: 1.0 KB ladder with sizes denoted in kilo bases (kb). (Caution, intensity of bands are not represented in this question!)

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