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1 A particular column has a length of 10 cm, plate height H of 0.004 cm, and so a plate number N of 2500. What is the effect of using a 20 cm long,...
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\‘.,- .1 A particular column has a length of 10 cm, plate height H of 0.004 cm, and so a platenumber N of 2500. What is the effect of using a 20 cm long, but otherwise identical,column, with the same mobile phase and flow rate, on: (a) the retention time of an analyte, am (b) the capacity factor of the analyte, k’ A (c) the separation factor between A and another analyte B, a“ (d) the width at the baseline of the peak due to A, WA.