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1. How much 4X SDS-PAGE sample buffer do you need to add to 10 ul of a cell extract to run it out on an polyacrylamide gel?

4. You have 20 ml of a protein sample in a 10% (w/v) suspension of sodium sulfate. How many rounds of dialysis (in a total volume of 1 liter) would be needed to reduce the salt concentration to 1 mg/ml or less?

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