1. How will we avoid permanently altering the electron micrograph image file in ImageJ? (2 points) 2.What is a fiduciary?

1.  How will we avoid permanently altering the electron micrograph image file in ImageJ? (2 points)

2.What is a fiduciary? (2 points)

3. What 3 types of modifications are you going to make to the electron micrograph in ImageJ? (3 points)

4. a. How many structures must you identify and label? (1 point)

    b. Where might you find pictures to use as references? (1 point)

 5.   How many structures must you measure? (1 point)

6.   a. What are the nuclei whose intensity we are measuring stained with? (1 point)

            b. What is the intensity proportional to? (2 points)

7.  Why do we use integrated density rather than area or mean density of the nuclei? (3 points)

8.  For what completely normal reason might some cells in an otherwise identical population have twice as much DNA as others? (3 points)

9.  When you set a threshold, what is the consequence if the images of two nuclei merge into one? Note: this is NOT the answer to the preceding question! (2 points)

10. Each wavelength corresponds to a different (spectral) color; thus, there are an infinite number of colors between 400 nm and 700 nm, our visual range. Furthermore, most objects have colors which are mixtures of different spectral colors - for example, purple results from a mixture of red and blue light.

      a. By what common mechanism do color TVs and computer monitors, color                       film, & cheap color printers produce the huge range of colors that they do?               Hint: your eye senses the infinite number of colors by a similar                                                    mechanism! (3 points)

      b. How much more information is encoded in a color image than in a grey scale    one? Explain. (2 points)