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1. Suppose you have two organisms in a mixture and organism a is 1000 times more abundant than organism b. will you be able to isolate organism b
1. Suppose you have two organisms in a mixture and organism a is 1000 times more abundant than organism b. will you be able to isolate organism b using the spread plate technique?
2. To get isolated colonies on a plate, only about 300 cells can be in the inoculum. What will happen if the cell density of the inoculum significantly exceeds the number?
3.What is the primary negative consequence of not spreading the inoculum evenly over the agar plate?
4.E. coli and S. marcescens were inoculate using the spread plate technique on Plates A,B,C ,D but isolation for E. coli was observed on plate C and Plate B for S. marcesens. Should they have been isolated on the same dilution plate? Why or Why not?
