52. Which of one the following amino acids is not phosphorylated in the C-terminal domain of the RPBI subunit of RNA polymerase Il?

52. Which of one the following amino acids is not phosphorylated in the C-terminal domain of the RPBI subunit of RNA polymerase Il?

a) Phe

b) Ser

c)     Tyr

d)    Thr

e)     All of the amino acids given are phosphorylated.

53. Which of the following RNA products are least likely to be synthesized by RNA polyinerase Il eukaryotic cells?

a) noncoding RN As

c)     mRNA

d)    tRNA

e)     None of the choices given is a RNA polymerase Il product.

54. Which of the following statements regarding transcription only applies to prokaryotes and not to eukaryotes?

a)     A region of the promoter about 30 nucleotides upstream of +1 contains AT-rich base pairs.

b)     The RNA start site is designated +1.

c)     A region of the promoter about 10 nucleotides upstream of +1 contains AT-rich base pairs.

d)     Important sequence elements are frequently found downstream of +1.

e)     All of the choices given apply to eukaryotes.

61) The majority of transcription in eukaryotic cells is accounted for by:

a)     Synthesis of pre-tRNA by RNA POI Ill

b)     Synthesis of pre-mRNA by RNA POI Il

c)     Synthesis of pre-rRNA by RNA POI Ill

d)     Synthesis of pre-rRNA by RNA POI I

e)     Synthesis of pre-tRNA by RNA POI I

63)  You create the following  genetic constructs:

a.     A fusion protein containing the DNA binding domain of the bacterial repressor Rep2 and the transcriptional activation domain of the yeast transcriptional activator SUGAR

b.     A shortened protein containing only the DNA binding domain of the of the bacterial repressor Rep2.

c.     A plasmid containing a copy of the promoter for the yeast gene SUGAR2 (which is activated by SUGAR]), driving the LacZ reporter gene.

d.     A plasmid containing a copy of the promoter for the yeast gene SUGAR2 (which is activated by SUGAR l), containing the DNA binding site for Rep2 immediately 5' of the RNA polymerase binding region of the promoter, driving the LacZ reporter gene.

Which construct(s) must you deliver to yeast to cause a high level of [3-galactosidase expression in these cells?

a) i

b) iii

c) iv

d)     I and iii

e)     i and iv

64.  Which one of the following codons is capable of wobble base pairing?

a)     5'-CAC-3'

b)     5'-CAG-3'

c)     5'-CAA-3'

d)     All of these codons are capable of wobble base pairing.

e)     None of these codons are capable of wobble base pairing.