a) In ELISA washing between steps is critical. Suggest what would happen (i. the result) if, due to poor washing:

a)    In ELISA washing between steps is critical. Suggest what would happen (i.e. the result) if, due to poor washing:i)     Free antigen was present when the specific antibody conjugate was added

ii)    Unattached antibody-conjugate was present when the substrate was added.