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a) Partial acid hydrolysis yields tripeptides with the following amino acid sequences asdetermined by manual Edman degradation: HSE, EGT, DYS, TSD,...
a) Partial acid hydrolysis yields tripeptides with the following amino acid sequences asdetermined by manual Edman degradation: HSE, EGT, DYS, TSD, FTS, YSK.
(b)Dansyl chloride treatment yields H and K.
(c) Brief carboxypeptidase A treatmentyields one K per mole peptide (acarboxypeptidase removes one residue at a time from the C-terminus of apolypeptide).
(d)Chymotrypsin treatment yields two peptides (A and B) with molecular masses of 659D and 467 D, respectively, and a dipeptide composed of S and K.
Peptide A (1) Dansyl chloride treatment yields H. (2) Brief carboxypeptidase A treatment yields F and T.Peptide B (3) Dansyl chloride treatment yields T. (4) Brief carboxypeptidase A treatment yields Y and D. (e)Trypsin treatment results in no fragmentation of the polypeptide.
