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A research associate working for Intragene Therapeutics prepares an investigative PCR reaction to screen human tissue samples for specific mutations....
A research associate working for Intragene Therapeutics prepares an investigative PCR reaction to screen human tissue samples for specific mutations. He uses 55 ng of genomic DNA from one tissue sample in a single PCR reaction, to amplify a 1,246 bp fragment. The PCR protocol requires a final concentration for two primers of 1.5 uM each, and a final concentration of 180 uM for dNTPs, in a total PCR reaction volume of 65 uL. During the PCR process, the fragment is amplified for 33 cycles. He quantifies the amount of PCR products to be 74 ng/uL.
1. If his purified genomic DNA template stock has a concentration of 1.53 mg/mL, and he dilutes it 1:100, how many microliters of this dilution does he use? Answer to one decimal place.
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