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An overnight bacterial culture was diluted 10 5 times and 0.1 ml was plated onto an agar plate. After overnight incubation the colonies were counted...
An overnight bacterial culture was diluted 105 times and 0.1 ml was plated onto an agar plate. After overnight incubation the colonies were counted to be 265 colonies.
1What is the original concentration of this bacterial overnight culture?
2One ml was transferred from the 105 dilution tube above into 9mls, mixed well and then 0.1ml was transferred on a plate. 14 colonies were detected and counted. What would the concentration of the overnight broth culture using the data off this plate? Which count would you consider more accurate, the one from (a) or this (b) and why?
