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Assume you have a stock culture at 5 x 10^9 cells per ml and you wish to inoculate 1 liter of a fresh medium so that in 15 h the cell density will be 2 x 10^8/mL. Assume a generation time of 3.5 h. Wh
Assume you have a stock culture at 5 x 10^9 cells per ml and you wish to inoculate 1 liter of a fresh medium so that in 15 h the cell density will be 2 x 10^8/mL. Assume a generation time of 3.5 h. What should be the dilution? What size inoculum should be used?