Below is the mechanism for the enzyme enolase, which converts 2-phosphoglycerate to phosphoenolpyruvate.

Below is the mechanism for the enzyme enolase, which converts 2-phosphoglycerate to phosphoenolpyruvate.  

C. What is the significance of producing phosphoenolpyruvate considering that a major goal of glycolysis is to produce ATP? What is the name for this process? Which enzyme in glycolysis performs this reaction and what is its DG´˚? Recall:

phosphoenolpyruvate + H2O à Pi + pyruvate          DG´˚ = -61.9kJ/mol

ATP + H2O à ADP + Pi                                               DG´˚ = -30.5kJ/mol