CALCULATING G AND G The conversion of 3-phosphoglycerate (3GP) to 2-phosphoglycerate (2GP) is an important cellular reaction because it is one of the...

CALCULATING ΔGº´ AND ΔG

The conversion of 3-phosphoglycerate (3GP) to 2-phosphoglycerate (2GP) is an important cellular reaction because it is one of the steps in the glycolytic pathway that we will discuss in chapter 3.

                                   3-phosphoglycerate ↔ 2-phosphoglycerate

If the enzyme that catalyzes this reaction is added to a solution of 3PG at 25°C and pH 7.0, the equilibrium ratio between the two species will be 0.165:

                       K´eq = [2-phosphoghlycerate]eq / [2-phosphoglycerate]eq = 0.165

Experimental values for the actual steady-state concentrations of these compounds in human red blood cells are 61 µM for 3PG and 4.3 µM for 2PG.

(A) Calculate ΔGº´. Explain what this value means.

I tried to attempt this since ΔGº´ = -2.303 RT log K'eq