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QUESTION
Can you describe this process?
Can you describe this process?
To move the shRNA expression units from pSilencer
into pUMVC3 vector, PCR primer was used to change
the BamHI sites to SalI site (BamHI to SalI primer, 50-AAGTCGACTGCTGTTGACAGTG-30),
PCR amplification with F seq primer (50-AGGCGATTAAGTTGGGTA-30), and the resulting PCR fragments were digested
with SalI and NotI and inserted into the SalI and NotI sites of pUMVC3.
