Consider issues related to a high throughput screen in 384-well plates to find inhibitors of the enzyme Factor XIIa. A) Identify a suita ble...

Consider issues related to a high throughput screen in 384-well plates to find inhibitors of the enzyme Factor XIIa.

A) Identify a suita ble fluorogenic peptide for this assay.

B) Assuming Km=18 uM and kcat= 0.53 s^-1 under the buffer conditions of the assay, calculate the time needed for 50% conversion of substrate if reactions are run at:

[S(t=0)] = 1.8uM

[E(t=0)] = 7nM

C) Calculate the time needed for 50% conversion of substrate if a competitive inhibitor (K1= 1uM) is used at a concentration of 5uM