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For the following questions, restriction enzyme digests were performed on a plasmid and the following size fragments were obtained: Sal 1:2 kb 5.1...
Q1. For the following questions, restriction enzyme digests were performed on a plasmid and the following size fragments were obtained:
Sal 1: 0.2, 1.2 kb & 5.1 kbTuf1: 3.1kb &3.4kbSal 1, Tuf 1 double digest: 0.2, 0.3, 0.9, 2.2 and 2.9 kb
•Station 1
- a) How many total kb is the intact plasmid?
- b) How many times does Sal 1 cut the plasmid?
- c) Draw a map of the plasmid with all of the Sal 1 and Tuf 1 recognition sites clearly labeled. Indicate the relative position of the sites by labeling the distance between each site in kb.