General Biology Lab I UC Berkeley Extension MCELLBI X15.1A-021 Overview Analysis of bacterial transformation with pGLO GFP purification (step 1) DNA...

1. What is the purpose of lysozyme in the GFP purification step 2?
2. Why is Hydrophobic Interaction Chromatography chosen to purify GFP? Explain.
3. How can you confirm the purification of GFP from a crude mixture after performing Hydrophobic Interaction Chromatography? Explain.
4. Sex-linked genes exhibit unique patterns of inheritance. Assuming there is one X-linked recessive trait that can cause a disorder in people. If the mother is the carrier (XBXb)and the father is normal (XBY) (Note: B is the dominant allele; b is the recessive allele), what is the inheritance pattern of this X-linked recessive trait in the next generation? Use Punnett square to show your answer. Explain this inheritance pattern. (10 points)
5. What is the role of an antibiotic resistance gene in the plasmid DNA? (10 points) (Hint: how can it help for bacterial transformation?)
6. What is the purpose of heat shock during bacterial transformation? (10 points)
7. Assuming you are doing restriction enzyme (EcoRI) digestion for your plasmid DNA followed by gel electrophoresis. You notice three distinct DNA bands (fragments) in your agarose gel. Is the DNA fragment closer to the positive electrode the shortest or the longest? Explain. (10 points)
8. The blood type O- is the universal blood donor and the blood type AB+ is the universal blood recipient. Explain the reasons. (10 points) ("-"= Rh- and "+" = Rh+)
9. Fill out the table (MCELLBI X15.1A-021 assignment 2 table.xlsx) to briefly summarize the key steps for GFP purification. (10 points)

Hi , I need very detailed and complete answers (not only one sentence response) to the following questions and no tutoring is needed.

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