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Hello, I am looking for someone to write an essay on The effect of manuka honey on elastase production by P. aeruginosa. It needs to be at least 2250 words.Pseudomonas species tend to be very resistan
Hello, I am looking for someone to write an essay on The effect of manuka honey on elastase production by P. aeruginosa. It needs to be at least 2250 words.
Pseudomonas species tend to be very resistant to most of antimicrobial therapies. During P. aeruginosa infections, P. aeruginosa elastase is released. This is a zinc metalloproteinase released during the process of infection. Any dead wound that is perfused allows multiplication of bacteria to multiply and elaborate an array of virulence. This factor can slow the process of healing in a wound. Protection of P. aeruginosa in the wound occurs through host immune effectors. This can grow at very sufficient levels to elaborate toxins that break down host factors. P. aureginosa has shown potential inactivation of human plasma alpha 1- proteinase. Favorable conditions of 25 degrees in temperature at a molar ratio of E/I= 1: 100 shows potential deactivation within an hour (El-Sukhon, 1994). The factors in target at this point are the fibrin and collagen molecules which have the mandate of healing issues and regeneration leading to complete healing of wounds. For this, purpose many scientist view pseudomonas as a delaying factor in the process of healing in the wounds. These syntheses are required for the synthesis of the auto inducer and the molecules which are linked with it like lactones and also homoserine. The pathogenesis linked with chronic infection along with the importance linked with quorum sensing remains unknown. P.aeruginosa is basically isolated from the patients of CF and the patients producing auto inducers and also the production is linked with CF sputum which is linked directly with the aeruginosa biofilms. The auto inducers are present in lung tissue of the infected mice especially with the P.aeruginosa. Many scientists have experienced honey used to heal wounds. The main function of honey is the stimulation of cytokine production by the monocytes present in the body of the organism. The cytokine production is accelerated hence leading to tissue repair and healing of wounds. The viscosity of manuka honey provides a moist environment, which helps in providing the protective barrier. In addition to this, honey has mild acidity and low levels of hydrogen peroxide stimulate the healing process. Studies related to this indicate that strong solutions of honey and sugar continue to inhibit microbial growth due to the presence of high osmolarity potentials (Lusby, 2005). The presence of high levels of hydrogen peroxide in manuka honey provides a good base for healing of wound and it activity in strains of P. aeruginosa. The experimental results show manuka honey having the potential production of broad-spectrum antibacterial activity. This process takes place by honey removing malodor. The anti-inflammatory activity of the manuka honey helps in reducing edema and consequently exudation and minimizing scarring. This process has the potential of stimulating the growth of granules. The granulation in addition to growth of epithelial tissues shows significant improvement in aiding the process of healing. The experiment shows that manuka honey has minimum inhibitory concentration of around 6% against P. aeruginosa strains. Further research by Nzaeko and Hamdi (Nzaeko BC, 2000) indicate that strains of E. coli and P. aeruginosa show inhibition levels of up to 40%. However, our study shows a significantly lesser percentage but the theory and conclusion is relatively common. P. aeruginosa produces an enzyme elastase, which has been shown to be one of the main virulent factors of these bacteria.
