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I need help creating a thesis and an outline on Comparative Analysis of Multi-Drug Resistance Gene Expression Level. Prepare this assignment according to the guidelines found in the APA Style Guide. A
I need help creating a thesis and an outline on Comparative Analysis of Multi-Drug Resistance Gene Expression Level. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstract is required.  .The pharmacological importance of the P-GP is based on three aspects of P-GP behaviour: (1). due to its broad substrate specificity and wide distribution in cells, it alters the behaviour of its substrates. (2) a number of clinically important compounds are known to alter the behaviour of P-GP eg. cortisol, erythromycin act as inhibitors while insulin, rifampin as inducers. (3) many clinically used compounds are known to be transported by P-GP. Thus a number of drug-drug interactions which remained unexplained till now are being found to be due to P-GP activity. Also in non-cancerous cells, the efficacy of drugs limited by P-GP can be improved by modifying the levels or activity of P-GP eg., P-GP is being examined as the reason for the resistance of human immunodeficiency virus (HIV) to antiretroviral agents (Schrager, 1998).To study the genomics and expression of MDR1 several methods can be used, however, detection at mRNA level is highly effective, sensitive and reproducible. In the present experiment, RT-PCR (reverse transcriptase-polymerase chain reaction) is being used to assess the levels of expression of P-GP in breast and cervical carcinoma cell lines and to make a comparative study for the two. mRNA is isolated from HeLa (cervical carcinoma cells) and MCF-7 (breast carcinoma cells). A cDNA copy of this mRNA is now made using the enzyme reverse transcriptase and PCR is done with the cDNA.
2 Oligonucleotide primers- short single-stranded DNA molecules that bind by complementary base pairing to opposite strands of template DNA, at either end of the DNA to be Primers are in vast excess to DNA to be amplified. They are oriented with their 3’ends facing each other so that the synthesis by DNA polymerase (which catalyzes growth in 5’®3’ direction) extends across the segments of DNA between them.
