I need some assistance with these assignment. plasmid analysis Thank you in advance for the help!

I need some assistance with these assignment. plasmid analysis Thank you in advance for the help! The blue plasmid DNA did not contain any of the restriction sites for Hind III or Sac I. Introduction Plasmids are the extra chromosomal DNA molecules which are mostly double –stranded, circular and covalently closed molecules, varying in size from 1 kb to 200 kb. (Sambrook and Russell 2001). They are found in many bacterial species. They replicate independently and use a variety of mechanisms to maintain their copy number. They contain the gene codes for the enzymes that are important for the bacterial hosts. The plasmids act as vectors in the molecular biology experiments. The vectors are the carrier DNA molecules into which the foreign gene of interest is inserted and expressed in the host. This DNA is now called recombinant DNA (Roberts and Murray 1976). This recombinant DNA is able to express the Foreign DNA in the bacteria. These vectors replicate inside the host cell along with the inserted DNA. These vectors are of two types: expression vectors (expression of the cloned gene to give the desired protein) and cloning vectors (produce millions of copies of cloned DNA) (Sambrook and Russell 2001). Restriction endonucleases are the enzymes that cut the DNA at the specific sequences. There are about 200 different restriction enzymes (Siwach and Singh 2007).