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QUESTION
If we you have two proteins in a mixture, one with a pI of 4.5, one with a pI of 9.5. In order to prevent these molecules from interacting would you
If we you have two proteins in a mixture, one with a pI of 4.5, one with a pI of 9.5. In order to prevent these molecules from interacting would you use high salt or low salt buffer and why? and what if you want to encourage interactions?
