Need an argumentative essay on Biochemical analysis of blood. Needs to be 4 pages. Please no plagiarism.Download file to see previous pages... If total proteins need to be evaluated, serum is therefor

Need an argumentative essay on Biochemical analysis of blood. Needs to be 4 pages. Please no plagiarism.

If total proteins need to be evaluated, serum is therefore the ideal choice. Liver function can be appropriately evaluated by measuring the total protein and albumin in the plasma sample and if a low concentration is detected, that means liver functioning is compromised (Gerardo 1998). Therefore, the total protein and albumin levels have been tested in this particular patient to evaluate his liver function. The level of albumin present is also an important marker of the state of osmotic regulation in the bloodstream as its deficiency can lead to oedema (known as dropsy, the medical term for fluid retention in the body). Oedema occurs as a result of certain health conditions, such as heart failure or kidney failure (NHS 2012). Total protein can be measured by the Biuret method. The method is based on the reaction between peptide bonds, imines and the carbonyl moiety in blood proteins, and a colour change results after reaction with Copper ions in an alkaline solution. The colour intensity can be measured by a spectrophotometer at 550nm wavelength and total protein can be estimated by comparing the value of obtained readings with that of standards. Another method of measuring proteins in a serum sample is by ‘Serum Protein Electrophoresis’ which elucidates levels of individual protein fractions in blood, such as albumin, ?, ?, and ? globulin. This provides a broad hint of the relative proportion of each protein fraction within the patient’s total serum. Cellulose acetate is used as a support medium in the electrophoresis tub. The negatively charged protein molecules on the cellulose acetate move towards the positively charged end, when electrical current is passed through the solution. The movement of the molecules depends on the size and charge taken up by each type of protein (Kaneko et al, 2008). Bilirubin a breakdown product of RBC (Red Blood Cells/Erythrocytes) disintegration, is another metabolic product whose level in the serum can assist in diagnosing liver malfunction. Liver is the site where bilirubin breakdown takes place and in case of liver malfunction, excessive bilirubin enters the bloodstream. Normal level of bilirubin in the blood as detected by the direct bilirubin (conjugated) estimation method is between 0 to 0.3 mg/dL, and as estimated by the total bilirubin method is 0.3 to 1.9 mg/dL (NIH, 2012). Any excess than these values is indicative of liver disease. This practical aims to recognize the importance of biochemical analysis of blood proteins in detecting diseases and carry out standardized clinical measurements of serum proteins in a blood sample obtained from a patient. Material and Methods: Patient's details Surname: OKEREKE Forename: JERMAINE DOB: 23/01/1950 URN: X855210 Information of: quality control used in SPE. Control: Kentro lot no: 109177702 expiry: 2012-06. Randox: lot no: 4674 expiry: 2014-03 Jermaine Okereke, a 62 year old male, was the patient from whom blood samples were taken. Dr. Siva, the attending physician had recommended several tests for this patient. The tests done in this practical were: 1. Serum Protein Electrophoresis 2. Total Protein by Biuret Method 3. Albumin by Bromcresol Green Binding, and 4. Bilirubin by Jendrassik and Grof Method. Serum Protein Electrophoresis: The tank was prepared by adding barbital buffer in two reservoirs. Filter paper wicks were used at both edges of the bridge.