Need an argumentative essay on The topic in the article. Needs to be 3 pages. Please no plagiarism.has been designed with the purpose of showing that the inhibition of auditory transmission, both in p

Need an argumentative essay on The topic in the article. Needs to be 3 pages. Please no plagiarism.

has been designed with the purpose of showing that the inhibition of auditory transmission, both in pre-synaptic afferents and post-synaptic inter-neurons, happens in coordination with the pattern of the song and/or sounds that they produce.

The experiments were performed to examine the activity of the inhibitory omega 1 neurons (ON1) and the auditory afferents in response to acoustic stimuli in three basic types of singing: sonorous or chirping, silent and lastly, fictive. A colony of Gryllus bimalculatus (male, adults) at temperatures of 18-22C was taken and fixed in a standing position allowing free wing movement, but the left wing later removed so as to allow silent singing only. Insect saline was used to bathe the nervous tissue that was exposed, while an acetylcholine esterase inhibitor was used to elicit singing by pressure injection into the anterior cerebrum.

For fictive singing, the acoustic stimuli that were generated via the software Cool Edit 2000 had a frequency of 4.5 kHz with 2-ms ramps of rise and fall, duration of 21 ms, intervals of 42-ms and r.m.s. of 100-dB SPL, mimicking that of natural singing. During singing, a sequence of duration 8 ms, short interval of 15 ms and r.m.s. of 75-dB SPL was also used in order to check the time of the neuronal response. The amplitude of the sound was calibrated using a Bruel and Kjaer measuring amplifier along with a microphone that was placed right next to the crickets’ ears, which are located on their forelegs.

0.5 M LiCl and 5% Lucifer yellow was used to stain and record the auditory neurons. This was followed by conventional processing of ganglia and identification of the stained neurons under UV fluorescent microscopy. A suction electrode was placed to record the motor activity on the mesothoracic nerve 3A, while an optoelectronic camera was used to monitor the movements of the wings. All recorded data was then sampled at a rate of 10 kHz per channel and analyzed using Microsoft Excel 2000 and