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Need an research paper on biomedical uses of biotechnology: flow cytometry and gel electrophoresis. Needs to be 9 pages. Please no plagiarism.
Need an research paper on biomedical uses of biotechnology: flow cytometry and gel electrophoresis. Needs to be 9 pages. Please no plagiarism. Flow Cytometry Analytical flow cytometry (AFC) is used to assess the biochemical composition of cells using an optical scanner in the assessment of individual cells as they are screened individually at a rapid rate (approximately 100 cells per second) through an optical scanner (Boddy et al 2001. Givan 2001). This methodology permits quantitative measurements of molecular components within each cell individually (Davey & Kell 1996. Givan 2001). An important medical use of AFC is the identification of specific strains of infectious bacteria in infected cells obtained from patient biopsy (Boddy et al 2001).
Once the pathogen is identified, AFC is also used in the therapeutic assessment of antibiotics on .the clinical course of infection by examining patient cells by AFC post-treatment. In this regard, AFC is an important biomedical tool in the assessment of parameters of clinical sensitivity and resistance of specific bacterial strains to specific therapeutic regimens (Davey & Kell, 1996). In addition, AFC can be used to measure the cellular DNA and protein concentration and the activity of specific .enzymes (Roederer 2001).
The capability of the AFC to assess molecular content within individual cells involves the use of fluorescence measurements to characterize the biochemical components of cells. In this technique, fluorescent probes are applied to specific cell molecules which are then assessed via the detection of optical excitation patterns emitted by these optically labeled cell components. (Shapiro 2003). These optical excitation patterns emitted by the fluorescently labeled cell components can be used to pinpoint specific .types of molecules and to quantitate their concentrations within the cell (Shapiro 2003). This accuracy is facilitated by the standardization of controlled flow by means of hydrodynamic focusing methods (Shapiro 2003). .
