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******* Bacteria *** ***************** **************** ** ************************** **** ************ ******* *** primary ******* is ** ******** unknown ******** from * ***** ******* The ******* ** ***** ** *** ******* ***** ** ******* ******** ************** can **** ** *********** the ********* ******* of * ************ ******* source *********** *** *** ********* ** dependent ** the ********* ******** **** *** present ** the *********** ******* ******** ************** ** * ********** and ******* ******* **** involves ********** ***** *** ********* ******** are ************** separated that are ******* ****** the ******* ******** culture Physicians ******* **** *** ******** process Ideally *** *** ******* *** ********* ***** the ******** and instructions **** *** instructor in *** ************** ** ***************** and MethodsThe materials **** ** *** ********** ****** Bunsen ******* Inoculation ************* Nutrient **** ********* peroxide)1 *** **** ****** Citrate **** ****** Grams ***** *** –crystal ****** ******** red iodine decolorizer4 *** ********** Microscope6 Slides7 **** (for test ****** *********** MicropipettesThe ***** **** **** ********* ****** the ******* ** unknown *** identification ****** Gram ***** tests2 ******** ****** Gas **** ******* tests5 ******** testsGram ***** ******* mixed ******* *** ******** to ******* the identification processThe ******* **** *** course ********** ****** was followed ** aid ** identificationA ****** was **** ** *** ******* culture ** a ********** *** **** ***** *** * streak ****** was ** *** *** manual ********** *** ****** method *** ** ******* *** ****** of *** unknown ********** morphology *** ******** after *** ********** *** ****** ** *** ****** *** gram stain ******* *** conducted **** *** later ******** ** *** ******* ******* of *** gram ******* bacteriaAfter *** gram reaction ******* there **** ******** biochemical ***** **** *** *********** ***** performed were taken **** ******* identification ****** present ** *** *** ****** *** ********** ** the ******* culture was ***** ** ***** ************** ********** ** the unknown ***** **** **** subjected ** catalase testsCatalase ******** process involved the addition ** * *** ***** ** ******** ******** ** *** ******** ******* Upon ******** ***** *** ** observation ** *** cultures that ******* ****** during ******** ******** ************ *** ******** ****** ** used ** an ********* environment Culturing ******** **** **** ** grow or **** die ** *** ****** ******** ** **** ** *** *** **** *** gas *** has * ****** and an ********* ***** ** within ** ******** *** *** *** *** of an ********* ** ********** ** it tells the presence ** ******* of oxygen ********* **** solution *** used *** ********* ** **** ******* *** placed **** *** *** to **** *** the ********** * change ** ***** shows **** ********* ********** *** ************** TestThe process ** used ** *** differentiation ** *** gram-negative ******* **** *** *** ** * *** ****** ********* ** *** slant *** **** **** and forth **** * ***** inoculum *** ***** inoculum *** ****** **** *** center of an isolated colony ********** *** aerobically ********* ** ************ ** **** *** * ******** ** ******* ************ ****** ** observation was later ************ ******** *** *** ** * *** manual * prepared ********* **** ****** ** a test-tube *********** *** **** **** ** * ******** **** ***** * single stab at *** ****** was prepared **** *** ***** of ******* ****** ********** ** 370c ** ****** ******** *********** ** ********* ** 1 **** *** **** ***** ******* **** takenResultsOn *** ********** ** *** **** ***** tests ** was evident that ***** **** unknown bacteria **** ******** **** **** rods **** was an ********** that *** gram ***** *** ******** *********** the appearance ** *** ****** rods *** ** ********** **** *** ******* ******** *** **** ***** *********** biochemical tests ** ******** ****** ******** and ******** ******* ** was concluded that *** ******** ******** ******** were *** ****** ********* **** *** based on the fact that **** ******* ***** ****** Contrastively the ******** ******** ******** **** ********** as ****** ******** ** **** ** ******* and do not **** respire using ****** ******* the ******** ******** ******** *** ** bubble formation ******** ******** ******** ** the other hand did *** ******* ********* the **** ** *** *** *** ***** *** ********* *** ******* **** **** **** ********* The *** ****** ********* an ********** of oxygen ***** **** *** *** ********* ********** being achieved *** ********* in the ********** *** ** **** ** *** *********** *** *********** ** ****** results *** the ******* **** ******** *** * **** blue *********** an ********** ** *** ********** *** ****** ** *** test-tube was the ********** ** * ***** ***** * **** ** *** ****** ************** *** **** **** *** **** against *** ***** *** **** **** was **** ** check *** ******** ********** *********** were ******** as ******* that **** ******** ** the **** **** *** ****** had ******* margins that **** *** medium transparent **** was ** contrast to the ****** ******** **** *** ******** *** *** hazy growths The ******* spread ** *** ****** ****** ** opaqueTestObservationResultGram *********** ******** ***** ************************************* ********** ************ ****** *** PresentCitrate ********* ******* ********* ** blueCitrate presentMotility ************ growths to ****** an opaque *********** presentDiscussionIdentifying ** organism genus requires **** careful ******* is taken to ********* *** ******* bacterial ******* In *** ********** * hypothesis was made ***** *** bacteria ***** ** *** ******** to fall ***** *** ************** ***** **** *** Gram ***** ******* ** *** **** the ******** mixture *** both *** **** ******** and **** negative results Gram-positive ******** **** a **** **** **** ** ******** ** ************* ******** ** ** enzyme *********** *** neutralization ** ***** ***** as **** ** *** ********* ******* ** ******** peroxide ******** **** initiates the ********* of ******** peroxide **** water *** ****** ******** *** catalase ******** ******* there are those that ** *** have ******* respiration which is ****** ** ******** ******** **** *** Gas *** ******* it *** also ******* **** bacterium cannot **** ** ****** ******* ******* ***** *** *** ** * carbon source by ** ******** *** ********* **** ******* ******* ******* ****** citrate which ********** ******* **** ********* **** ** **** as ****** **** Oxaloacetic **** ** **** hydrolyzed to ******* **** *** ****** ******* In **** ****** ******* ** released **** ** ****** with *** ****** ** create ** ******** ****** **** leads ** * change ** *** ********* The ****** in *** ***** to **** *** ********** ** ********** was ** ********** ** the positive ***** *** ******* ** the ******** **** there were ******** ****** *** non-motile * ****** ******** *** ****** direction *** **** ** ********** that are ******* *********** *** away **** the repellants ** *** apparent **** *** ******** could ***** *** *********** ********** *** * ****** ************************ processStaphylococcus ****** ** ************* ***** **** ****** in the **** of ******** ** *** **** ***** Besides ***** ****** ** ******** ******** ** indication ** the ******* ** ******* ******** ****** **** the aid ** the ****** ********* ******** hydrogen ******** ** ***** *** ****** ** ** *** characteristic ** *** staphylococcus **** ***** ** ************** ******* ************* and *********** ** can **** be differentiated **** ***** organisms **** ** ***** *********** ** **** ** ***** saprophyticus **** ********* on *** citrate tests ***** ** ****** in both peptone *** distilled ***** a ************** **** ********* ** **** other ********* ************* vibrios are *********** ** ********* ***** but **** the motile *************** ** *** ******* ***** ************ This ********** *** differences under *** ******** testReferencesBergey’s *************** System **** ********* ***** ******* ************ ********* 2016-12-03Lab ***************** M * ****** ******* **** ******** ******** **** **** *********************************************************************************************