Provide a 12 pages analysis while answering the following question: Mechanisms of Protein Folding In Vitro. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstrac

Provide a 12 pages analysis while answering the following question: Mechanisms of Protein Folding In Vitro. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstract is required. The scientific joy of those who have seen the new light is accompanied by the dismay of those whose way of life has been changed forever. The publication of the first structures of proteins at atomic resolution fifty years ago astounded and inspired scientists in every field, but caused others to flee or scoff. Advance and subsequent paradigm-shifting breakthroughs and impacts on the field of protein folding have met with some resistance before universal acceptance” (Fersht 2008).

The past twenty-five years have revealed substantial breakthroughs in understanding and effective application of ‘protein folding mechanisms’. Radford (2000) of the University of Leeds (Leeds, UK), School of Biochemistry and Molecular Biology, also meaningfully share on the motivating scientific dynamics of biophysics and protein folding. “… We have reached an exciting stage with consensus beginning to emerge that combines both theoretical and experimental approaches. Furthermore, new fields and disciplines have emerged, including in vitro protein folding and the study of protein misfolding diseases.&nbsp.In today’s post-genomic world, understanding protein folding has never been more important. The topic has wide-ranging impacts in fields from structural biology to materials science” (Radford 2000).

During biomolecular and biophysical experimentation in the Matysiak Lab (University of Maryland), the Biomolecular Modeling Group conducts modern theoretical and computational studies that construct effective scientific support for experiments toward a thorough description or characterization of protein folding and dynamics mechanisms. Course-grained in vitro protein models for the characterization of folding landscapes is used as descriptions of protein folding processes and frameworks for the interpretation of research study results. (Fig. 2.1).

The Biomolecular Modeling Group explains the purpose of in vivo modeling. “Recent advances in protein modeling and development of methodologies are allowing theoretical and experimental synergistic characterization of folding landscape. The protein folding landscape can provide us with a theoretical framework that can also be used to characterize the physical and chemical factors regulating protein misfolding mechanisms” (Biomolecular Modeling Group 2013). The Biomolecular Modeling Group further clarifies their recent experimental goals. “Our group develops models and methods to quantitatively map the route from the unfolded protein to the correctly folded state on a protein-free energy landscape. The main goal is to investigate how certain perturbations such as mutations or changes in environmental conditions may have dramatic disease-triggering changes on the landscape” (Biomolecular Modeling Group 2013).