Provide a 7 pages analysis while answering the following question: Molecular Genetics. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstract is required.

Provide a 7 pages analysis while answering the following question: Molecular Genetics. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstract is required. With the help of a molecular ladder which can be obtained by digestion of the λ DNA with HindIII, the size of the plasmid isolated can be approximated. Lambda DNA on digestion with HindIII breaks into seven fragments of sizes 23.130 kb, 9.416 kb, 6.557 kb, 4.361 kb, 2.322 kb, 2.027 kb, and 0.564 kb. As because the concentration of the digested lambda DNA was less all the digested products could not be visualized i.e. only three bands of the digested DNA which was of larger size could be visualized whereas the smaller fragments could not be visualized. Comparing the band of the plasmid isolated and the bands obtained by the lambda DNA digestion (molecular ladder) it can be approximated that the size of the plasmid isolated is around 3.5-4 kb as the band of the isolated plasmid is further down the third band obtained and would have corresponded to a region between the fourth and fifth bands by digestion of the lambda DNA. The other who ran the gel using the plasmid that they had extracted also estimated the size of the plasmid using the same procedure.

The size of the plasmid isolated by Group H can be analyzed by the same procedure. The plasmid isolated by Group H corresponds to the third band of the lambda digested product can be approximated to a size of around 6kb.

The lambda fragments joined on treatment with a ligase to form a larger molecule of the size of around 48.337kb. The ligation procedure was successful as it is very well visualized by comparing the mobility of the ligated product with that of the fragments. The ligated product being larger in size moves with a lesser velocity.

The gel that we used for the procedure was&nbsp.made of agarose and it was dull in appearance as it does not fluoresce (glows) and it provides a perfect background for the visualization of the DNA bands which fluoresces. The DNA which was loaded in the well was found to move towards the positive end of the electric field. However, the final result was not something that I anticipated. The gel was expected to show the presence of the DNA fragments in the form of bands. These bands would be located at regions where the DNA molecules migrate (towards the positive end of the electric field) under the influence of the electric field depending on the size as well as on the shape. The gel showed above-expected results.&nbsp.&nbsp.