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QUESTION
Step 6: Ligation of purified RT-PCR product into plasmid vector. Next we will mix the purified RT-PCR product with a linear plasmid vector,...
Step 6: Ligation of purified RT-PCR product into plasmid vector. Next we will mix the purified RT-PCR product with a linear plasmid vector, pGEMT-Easy. T4 DNA Ligase enzyme will catalye the formation of phosphodiester bonds between the fragment and vector DNAs. What features of each molecule facilitates their interaction?
