Successful cloning of the annealed primers into the pX459 plasmid will introduce a new NcoI cutting site. With the overall plasmid size of 9174bp remaining the same and the new NcoI site at position 2

Successful cloning of the annealed primers into the pX459 plasmid will introduce a new NcoI cutting site. With the overall plasmid size of 9174bp remaining the same and the new NcoI site at position 249, restriction enzyme digest with NcoI will result in ( ) fragments. The order of these fragments viewed on an agarose gel from the top (loading wells) to the bottom are the  ( ) bp fragment, followed by the ( )  bp fragment and the ( ) bp fragment at the last position.