Suppose you want to figure out what genes are involved in the production of proline (Pro) in a certain bacteria. (1) How would you go about designing...

Suppose you want to figure out what genes are involved in the production of proline (Pro) in a certain bacteria.

(1) How would you go about designing a mutant screen to do so?

(2) Suppose your screen identified 5 mutants that barely grew without being given Pro in the medium. How would you go about determining the number of different genes these mutants represent?