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The average molecular weight of a nu cleotide (bbase + deoxyribose + 1 phosphate group)) in DNA is 308g//mmol of 308 Daltons (DDa)). This number is...
3.. The average molecular weight of a nu cleotide (bbase + deoxyribose + 1 phosphate group)) in DNA is 308g//mmol of 308 Daltons (DDa)).. This number is the average molecular weight of A = 312.2 g//mmol G = 328.2 g//mmol C = 288.2 g//mmol T = 303.2 g//mmol
a.. Which plasmid was used for the transformation experime nt?? What is the size of this plasmid (iin kilo base pairs;; kbp))?? Ho w many bases is this??
b.. What is the molecular weight of one plasmid molecule??
c.. You used 25µl of 1ng//µµl plasmid DNA per transformation.. How many grams of plasmid DNA did you use?? How many moles of plasmid DNA is that?? How many molecu les of plasmid DNA is that??
d.. Assuming each transformed cell pick up one plasmid molecules during the transformation process,, what percentage of DNA molecules successfully entered and replic ated inside the competent cells?
