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To characterize the acidic sites on a new polymer biomaterial, you measure the charge of the polymer as a function of pH. The data are: A) How many
To characterize the acidic sites on a new polymer biomaterial, you measure the charge of the polymer as a function of pH. The data are:
A) How many acidic sites does each polymer have?
B) Use a Scatchard-type analysis to estimate how many different types of acidic sites exist, how many sites of each type a polymer contains, and the pKa for each type. Assume that the acid dissociation reactions are all independent.
[Hint: Scatchard analysis requires that the data be written for a binding reaction, rather than for a dissociation reaction. So the first step is to rewrite the charge vs. pH data into bound ligand vs. ligand concentration data.]
