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QUESTION
trypan blue was used to differentiate dead cells. isolated whole blood leukocytes live cells= 24 dead cell=11 viability=63% mononuclear cells (PBMC)...
trypan blue was used to differentiate dead cells.
isolated whole blood leukocytes
live cells= 24
dead cell=11
viability=63%
mononuclear cells (PBMC)
live cells= 297
dead cell=84
viability=77.95%
the cell number significantly increased? why this might be. this value was expected or not.
why an increase in trypan blue stained (dead) cells?
