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QUESTION
What is a potential complication in this experiment using a coupled product that contains a chiral amide?
- What is a potential complication in this experiment using a coupled product that contains a chiral amide? How would you determine if changes in the CD spectrum are just due to signals from the molecule or from the actual alterations in the proteins conformation?
- What is the difference between an excitation and an emission spectrum?
- Explain why quantum yield and the extinction coefficient are important factors to consider when using a fluorescent based method.
- What are the consequences if your drug binds too tightly to HSA? What if it doesn't bind to HSA at all? How might the binding affect the efficacy of your drug?
- When choosing a fluorescent probe for an experiment like this lab, what are two things you would want to consider?
- Would this assay be easily adaptable to a high-throughput screening method, commonly used in pharmaceutical companies? Why or why not?
- What kind of factors can affect the fluorescence of a compound?
- What are two advantages and two disadvantages to using a fluorescent based assay?
