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What would be the original concentration in a solution when we performed one 1 to 1000 dilution and two 1 to 2 dilutions then plated 100 microliters

What would be the original concentration in a solution when we performed one 1 to 1000 dilution and two 1 to 2 dilutions then plated 100 microliters of the final dilution on a plate and saw 80 bacterial colonies grow on it the following day? 

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