Which of the following experiments provided the first evidence that the amino acid sequence of a polypeptide chain contains all the information

Which of the following experiments provided the first evidence that the amino acid sequence of a polypeptide chain contains all the information required to fold the chain into its native, three-dimensional structure? A) When ribonuclease is treated with urea, it loses its catalytic activity B) When denatured ribonuclease is allowed to renature, it regains its catalytic activity C) When renatured ribonuclease is allowed to denature, it regains its catalytic activity D) Addition of mercaptoethanol causes ribonuclease to regain catalytic activity E) Addition of mercaptoethanol causes ribonuclease to lose catalytic activity