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QUESTION

Which of the following primer sequences has the highest Tm?

Which of the following primer sequences has the highest Tm?

A) 

AAATTTAAATTTAAATTT

B) 

GGGTTTAAATTTAAACCC

C) 

AAAAAAGGGGGGTTTTTT

D) 

GGGAAACCCTTTAAATTT

E) 

AAACCCCCCTTTGGGAAA

I have been going to different Tm calculators, to no avail (in my opinion)

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