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Which of the following primer sequences has the highest Tm?
Which of the following primer sequences has the highest Tm?
A)
AAATTTAAATTTAAATTT
B)
GGGTTTAAATTTAAACCC
C)
AAAAAAGGGGGGTTTTTT
D)
GGGAAACCCTTTAAATTT
E)
AAACCCCCCTTTGGGAAA
I have been going to different Tm calculators, to no avail (in my opinion)