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Write a 2 page essay on Immunological Techniques Assignment.Download file to see previous pages... Immunoelectrophoresis is not very sensitive to Ag-Ab ratio and can be used to detect light chains in
Write a 2 page essay on Immunological Techniques Assignment.
Download file to see previous pages...Immunoelectrophoresis is not very sensitive to Ag-Ab ratio and can be used to detect light chains in Ag excess. The length of the arcs gives the amount of immunoglobulin present which makes this process semi-quantitative. The process may be used to detect monoclonal proteins in both blood and urine. The radial immunodiffusion is a more widely used quantitative method and can be used to quantify immunoglobulins (including IgG subclasses) and serum proteins. However even though it is sensitive method the reaction time is long. Ouchterlony is also a qualitative method used to detect similarities between antigens. Just like RID the reaction time is long.
