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You discover a new eukaryotic RNAP. To study this enzyme you have developed an in vitro assay with an inhibitor of chain elongation,...
You discover a new eukaryotic RNAP. To study this enzyme you have developed an in vitro assay with an inhibitor of chain elongation, 3’-deoxy-5’[α-32P]CTP. You are surprised by the fact that no 32P is found in the transcribed RNA! What does this result tell you about the mechanism of the new RNAP?
You discover a new eukaryotic RNAP. To study this enzyme you have developed an in vitroassay with an inhibitor of chain elongation, 3’-deoxy-5’ [α-32P] CTP. You are surprised by thefact that...